Development of an analytical method for the determination of ciprofloxacin, diclofenac, acetaminophen, and carbamazepine by High-Performance Liquid Chromatography

A.J. Alvillo-Rivera; R.A. Guillén-Garcés; L.G. Treviño-Quintanilla; I.E. Villegas-Mendoza; C.D. Silva-Luna

Authors

A.J. Alvillo-Rivera UPEMOR, Universidad Politécnica del Estado de Morelos. Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Jiutepec, Morelos. C.P. 62550. Author https://orcid.org/0000-0002-6759-5227 (unauthenticated)
R.A. Guillén-Garcés UPEMOR, Universidad Politécnica del Estado de Morelos. Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Jiutepec, Morelos. C.P. 62550 Translator https://orcid.org/0000-0003-1257-352X (unauthenticated)
L.G. Treviño-Quintanilla UPEMOR, Universidad Politécnica del Estado de Morelos. Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Jiutepec, Morelos. C.P. 62550 Author https://orcid.org/0000-0002-6677-9630 (unauthenticated)
L.E. Villegas-Mendoza IMTA, Instituto Mexicano de Tecnología del Agua. Paseo Cuauhnáhuac 8532, Jiutepec, Morelos. C.P. 62550. Author https://orcid.org/0000-0002-1367-6558 (unauthenticated)
C.D. Silva-Luna IMTA, Instituto Mexicano de Tecnología del Agua. Paseo Cuauhnáhuac 8532, Jiutepec, Morelos. C.P. 62550. Author https://orcid.org/0000-0003-3645-8120 (unauthenticated)

Keywords:

Chromatography, emerging pollutants, pharmaceuticals.

Abstract

The drugs used in this study are commonly used in Mexico to treat joint pain (diclofenac), fever (acetaminophen), bacterial infections (ciprofloxacin), and epilepsy (carbamazepine). High-Performance Liquid Chromatography (HPLC) has been reported for the analysis of various compounds present in biological and environmental fluids. The objective of this study was to develop a rapid and simple method for determining the aforementioned medications in water samples. For this purpose, the HPLC method with ultraviolet detection at 277 nm, an isocratic mobile phase, and a quantification range of 1 to 50 μg∙mL^-1was used. The retention times were 6.49, 8.45, 9.56 and 8.56 minutes for diclofenac, ciprofloxacin, acetaminophen, and carbamazepine, respectively, at the same wavelength. Precision and accuracy exceeded 95%, and the total elution time was 12 minutes per sample. An analytical method for the determination of diclofenac, ciprofloxacin, acetaminophen and carbamazepine in water was developed and implemented. This method was sufficiently sensitive, accurate, and reproducible, with a degree of error of less than 5%, making it suitable for use in future studies.

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Development of an analytical method for the determination of ciprofloxacin, diclofenac, acetaminophen, and carbamazepine by High-Performance Liquid Chromatography

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