Assessment of the cytotoxicity of PCL and PLGA scaffolds with dental pulp stem cells for tissue engineering
Keywords:
Dental pulp stem cells, lactic-co-glycolic acid, poly - ε caprolactone, scaffolds, tissue engineering.Abstract
Materials of different nature such as poly - ε caprolactone and glycolic poly-lactic acid, have been used in regenerative medicine with human applications and dental pulp stem cells represent an attractive source of cells to be used in in vitro assays due to their easy accessibility, good cell viability and the fact that they are obtained from human third molars in a routine extraction.
Objective: To evaluate the in vitro cytotoxicity of PCL/PLGA scaffolds cultured with dental pulp stem cells.
Materials and methods: An experimental in vitro study was carried out evaluating PCL/ PLGA scaffolds in two portions (80:20 and 90:10, respectively) on which DPSC obtained from third molars were planted. The cell viability was evaluated at three times 3, 7 and 10 days by the MTT assay. Data analysis was performed with the Stata software version 14 and the Student's "t" test.
Results: Culture and characterization of dental pulp stem cells were positive for CD44 and Stro-1, the results show an increase in cell viability from day 3 in the control groups and both polymer mixtures. But there was a lower metabolic activity in the PLGA (90:10) compared to the PLC/PLGA (80:20).
Conclusions: PCL/PLGA 80:20 scaffolds showed greater cell viability at days 7 and 10 compared to the PCL/PLGA 90:10. It was also shown that neither mixture has cytotoxic effect on dental pulp stem cells.
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