Encapsulation of lipase from Aspergillus niger GH1 and evaluation of their disintegration In Vitro

Abstract

Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. These enzymes are naturally present in the human body and are essential to digest fats. In the present study, we focused on the encapsulation of lipases produced by Aspergillus niger GH1 fungus in an alginate matrix, using three concentrations of sodium alginate (2, 2.5, and 3.0 % w/v) and two concentrations of CaCl₂ were evaluated (0.1 and 0.2M) such as crosslinking agent. The results show that alginate provides a diffusion barrier which enables resist gastric conditions, so they can be released into the intestine in 28 minutes, where the enzymes hydrolysis triglycerides. The results showed that with 3.0 % alginate and 0.2 M CaCl₂ concentration, the morphological characteristics and enzymatic activity are the best results, thus being effective for encapsulating and releasing lipases.