Inmunolocalización celular de una enzima participante en la ruta de degradación del Naftaleno
Palabras clave:
Bioquímica, biorremediación, Inmunolocalización, naftaleno.Resumen
La contaminación por derrames de petróleo es uno de los problemas a los que comúnmente nos enfrentamos hoy en día, uno de los métodos que se ha planteado para la reducción de los contaminantes del suelo es el uso de biorremediación mediada por hongos, Mucor circinelloides es un hongo capaz de crecer y metabolizar residuos de petróleo como los hidrocarburos policíclicos aromáticos (HPAs), específicamente el naftaleno. Como una parte fundamental de conocer el proceso de metabolismo de los HPAs, en este trabajo se llevó a cabo la identificación de la presencia de la segunda enzima que participa en la ruta de degradación de naftaleno, la dihidrodiol deshidrogenasa (DD), su localización mediante anticuerpos marcados y su distribución mediante microscopía electrónica de transmisión, obteniendo que la enzima DD se encuentra localizada en el citoplasma celular en partículas electrodensas que son diferentes a los peroxisomas, estos hallazgos pueden ayudar a mejorar el panorama de biorremediación de compuestos recalcitrantes derivados del petróleo.
Referencias
[1] M. D. King, J. E. Elliott, and T. D. Williams, “Effects of petroleum exposure on birds: A review”, Science of The Total Environment, vol. 755, p. 142834, Feb. 2021, doi: 10.1016/j.scitotenv.2020.142834.
[2] L. Mondragón-Camarillo, S. R. Zaragoza, Ma. R. Mendoza-López, N. Cabirol, and M. Macek, “Recovery of Soil Protozoan Community Structure Promoted by M. sativa After a Strong Pulse of Hydrocarbon Contamination”, Water Air Soil Pollut, vol. 231, no. 6, p. 283, 2020, doi: 10.1007/s11270-020-04618-7.
[3] D. B. Gleeson et al., “Natural attenuation of legacy hydrocarbon spills in pristine soils is feasible despite difficult environmental conditions in the monsoon tropics”, Science of The Total Environment, vol. 799, p. 149335, Dec. 2021, doi: 10.1016/J.SCITOTENV.2021.149335.
[4] J.-W. Liu et al., “Surfactant-enhanced remediation of oil-contaminated soil and groundwater: A review”, Science of The Total Environment, vol. 756, p. 144142, 2021, doi: https://doi.org/10.1016/j.scitotenv.2020.144142.
[5] H. Luo, H. Wang, L. Kong, S. Li, and Y. Sun, “Insights into oil recovery, soil rehabilitation and low temperature behaviors of microwave-assisted petroleum-contaminated soil remediation”, J Hazard Mater, vol. 377, pp. 341–348, 2019, doi: https://doi.org/10.1016/j.jhazmat.2019.05.092.
[6] A. Dhaka and P. Chattopadhyay, “A review on physical remediation techniques for treatment of marine oil spills”, J Environ Manage, vol. 288, p. 112428, Jun. 2021, doi: 10.1016/J.JENVMAN.2021.112428.
[7] P. E. Ndimele, A. O. Saba, D. O. Ojo, C. C. Ndimele, M. A. Anetekhai, and E. S. Erondu, “Remediation of Crude Oil Spillage”, The Political Ecology of Oil and Gas Activities in the Nigerian Aquatic Ecosystem, pp. 369–384, Jan. 2018, doi: 10.1016/B978-0-12-809399-3.00024-0.
[8] A. Shah and M. Shah, “Characterisation and bioremediation of wastewater: A review exploring bioremediation as a sustainable technique for pharmaceutical wastewater”, Groundw Sustain Dev, vol. 11, p. 100383, Oct. 2020, doi: 10.1016/J.GSD.2020.100383.
[9] C. M. Quintella, A. M. T. Mata, and L. C. P. Lima, “Overview of bioremediation with technology assessment and emphasis on fungal bioremediation of oil contaminated soils”, J Environ Manage, vol. 241, pp. 156–166, Jul. 2019, doi: 10.1016/J.JENVMAN.2019.04.019.
[10] I. M. Gillespie and J. C. Philp, “Bioremediation, an environmental remediation technology for the bioeconomy,” Trends Biotechnol., vol. 31, pp. 329–332, 2013.
[11] N. Akhtar and M. A. Mannan, “Mycoremediation: Expunging environmental pollutants”, Biotechnology Reports, vol. 26, p. e00452, 2020, doi: https://doi.org/10.1016/j.btre.2020.e00452.
[12] A. Ratnasari, A. Syafiuddin, A. B. H. Kueh, S. Suhartono, and T. Hadibarata, “Opportunities and Challenges for Sustainable Bioremediation of Natural and Synthetic Estrogens as Emerging Water Contaminants Using Bacteria, Fungi, and Algae”, Water Air Soil Pollut, vol. 232, no. 6, pp. 1–23, Jun. 2021, doi: 10.1007/S11270-021-05183-3/FIGURES/5.
[13] S. Verma and A. Kuila, “Bioremediation of heavy metals by microbial process”, Environ Technol Innov, vol. 14, p. 100369, May 2019, doi: 10.1016/J.ETI.2019.100369.
[14] J. A. Mir-Tutusaus, R. Baccar, G. Caminal, and M. Sarrà, “Can white-rot fungi be a real wastewater treatment alternative for organic micropollutants removal? A review”, Water Res, vol. 138, pp. 137–151, Jul. 2018, doi: 10.1016/J.WATRES.2018.02.056.
[15] Y. Wang, S. Chen, J. Zhou, X. Fan, L. He, and G. Fan, “Enhanced degradation capability of white-rot fungi after short-term pre-exposure to silver ion: Performance and selectively antimicrobial mechanisms”, Science of the Total Environment, vol. 818, Apr. 2022, doi: 10.1016/j.scitotenv.2021.151672.
[16] A. C. del Álamo, M. I. Pariente, R. Molina, and F. Martínez, “Advanced bio-oxidation of fungal mixed cultures immobilized on rotating biological contactors for the removal of pharmaceutical micropollutants in a real hospital wastewater”, J Hazard Mater, vol. 425, Mar. 2022, doi: 10.1016/j.jhazmat.2021.128002.
[17] L. Palli, A. Gullotto, S. Tilli, D. Caniani, R. Gori, and A. Scozzafava, “Biodegradation of 2-naphthalensulfonic acid polymers by white-rot fungi: Scale-up into non-sterile packed bed bioreactors”, Chemosphere, vol. 164, pp. 120–127, Dec. 2016, doi: 10.1016/j.chemosphere.2016.08.071.
[18] Y. Ding, K. Cui, X. Liu, Q. Xie, Z. Guo, and Y. Chen, “Lignin peroxidase-catalyzed direct oxidation of trace organic pollutants through a long-range electron transfer mechanism: Using propranolol as an example”, J Hazard Mater, vol. 431, Jun. 2022, doi: 10.1016/j.jhazmat.2022.128544.
[19] Y. Lee, Y. Lee, and C. O. Jeon, “Biodegradation of naphthalene, BTEX, and aliphatic hydrocarbons by Paraburkholderia aromaticivorans BN5 isolated from petroleum-contaminated soil”, Scientific Reports 2019 9:1, vol. 9, no. 1, pp. 1–13, Jan. 2019, doi: 10.1038/s41598-018-36165-x.
[20] S. Kumari, R. K. Regar, and N. Manickam, “Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria”, Bioresour Technol, vol. 254, pp. 174–179, Apr. 2018, doi: 10.1016/J.BIORTECH.2018.01.075.
[21] A. Imam, S. Kumar Suman, P. K. Kanaujia, and A. Ray, “Biological machinery for polycyclic aromatic hydrocarbons degradation: A review”, Bioresour Technol, vol. 343, p. 126121, Jan. 2022, doi: 10.1016/J.BIORTECH.2021.126121.
[22] W. Wang, L. Wang, and Z. Shao, “Polycyclic aromatic hydrocarbon (PAH) degradation pathways of the obligate marine PAH degrader Cycloclasticus sp. strain P1”, Appl Environ Microbiol, vol. 84, no. 21, Nov. 2018, doi: 10.1128/AEM.01261-18/SUPPL_FILE/ZAM021188824S1.PDF.
[23] K. Nomura et al., “Identification of New Dioxygenases Able to Recognize Polycyclic Aromatic Hydrocarbons with High Aromaticity”, Catalysts 2022, Vol. 12, Page 279, vol. 12, no. 3, p. 279, Mar. 2022, doi: 10.3390/CATAL12030279.
[24] J. E. Rogers and D. T. Gibson, “Purification and properties of cis-toluene dihydrodiol dehydrogenase from Pseudomonas putida”, J Bacteriol, vol. 130, no. 3, pp. 1117–1124, 1977, doi: 10.1128/JB.130.3.1117-1124.1977.
[25] R. L. Camacho-Morales, V. Zazueta-Novoa, J. L. G. Casillas, E. A. Ballesteros, J. A. O. Bote, and R. Zazueta-Sandoval, “Purification and characteristics of an inducible by polycyclic aromatic hydrocarbons NADP+-dependent naphthalenediol dehydrogenase (NDD) in Mucor circinelloides YR-1”, Protein Expr Purif, vol. 97, pp. 1–8, May 2014, doi: 10.1016/J.PEP.2014.02.001.
[26] Y. Alvarado-Caudillo et al., “Presence and physiologic regulation of alcohol oxidase activity in an indigenous fungus isolated from petroleum-contaminated soils”, Applied Biochemistry and Biotechnology 2002 98:1, vol. 98, no. 1, pp. 243–255, 2002, doi: 10.1385/ABAB:98-100:1-9:243.
[27] K. L. Lee, H. R. Buckley, and C. C. Campbell, “An amino acid liquid synthetic medium for the development of mycellal and yeast forms of Candida albicans”, Sabouraudia: Journal of Medical and Veterinary Mycology, vol. 13, no. 2, pp. 148–153, Jan. 1975, doi: 10.1080/00362177585190271.
[28] A. Rosas-Arellano et al., “A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays”, Histochemistry and Cell Biology 2016 146:4, vol. 146, no. 4, pp. 421–430, May 2016, doi: 10.1007/S00418-016-1447-2.
[29] P. Sawulski, B. Boots, N. Clipson, and E. Doyle, “Differential degradation of polycyclic aromatic hydrocarbon mixtures by indigenous microbial assemblages in soil”, Lett Appl Microbiol, vol. 61, no. 2, pp. 199–207, Aug. 2015, doi: 10.1111/lam.12446.
[30] Sakshi and A. K. Haritash, “A comprehensive review of metabolic and genomic aspects of PAH-degradation”, Arch Microbiol, vol. 202, no. 8, pp. 2033–2058, Oct. 2020, doi: 10.1007/s00203-020-01929-5.
[31] R. H. Peng et al., “Microbial biodegradation of polyaromatic hydrocarbons”, FEMS Microbiol Rev, vol. 32, no. 6, pp. 927–955, Nov. 2008, doi: 10.1111/J.1574-6976.2008.00127.X.
[32] D. Miyazawa et al., “Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8”, Microorganisms, vol. 8, no. 1, Jan. 2020, doi: 10.3390/MICROORGANISMS8010044.
[33] H. Silva-Jiménez and R. Zazueta-Sandoval, “Intracellular fate of hydrocarbons: possible existence of specific compartments for their biodegradation”, Appl Biochem Biotechnol, vol. 121–124, no. 1–3, pp. 205–217, 2005, doi: 10.1385/ABAB:121:1-3:0205.
[34] H. Peidro‐Guzmán et al., “Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions”, Environ Microbiol, vol. 23, no. 7, pp. 3435–3459, Jul. 2021, doi: 10.1111/1462-2920.15166.
[35] J. N. Bateman, B. Speer, L. Feduik, and R. A. Hartline, “Naphthalene association and uptake in Pseudomonas putida”, J Bacteriol, vol. 166, no. 1, pp. 155–161, 1986, doi: 10.1128/JB.166.1.155-161.1986.
[36] B. Mohapatra and P. S. Phale, “Microbial Degradation of Naphthalene and Substituted Naphthalenes: Metabolic Diversity and Genomic Insight for Bioremediation”, Front Bioeng Biotechnol, vol. 9, p. 144, Mar. 2021, doi: 10.3389/FBIOE.2021.602445/BIBTEX.
[37] A. Choudhary, H. Purohit, and P. S. Phale, “Benzoate transport in Pseudomonas putida CSV86”, FEMS Microbiol Lett, vol. 364, no. 12, Jul. 2017, doi: 10.1093/FEMSLE/FNX118.
[38] V. Kumar and S. K. Dwivedi, “Mycoremediation of heavy metals: processes, mechanisms, and affecting factors”, Environmental Science and Pollution Research, vol. 28, no. 9, pp. 10375–10412, 2021, doi: 10.1007/s11356-020-11491-8.
[39] A. K. Rathankumar et al., “Surfactant-aided mycoremediation of soil contaminated with polycyclic aromatic hydrocarbon (PAHs): progress, limitation, and countermeasures”, Journal of Chemical Technology & Biotechnology, vol. 97, no. 2, pp. 391–408, Feb. 2022, doi: https://doi.org/10.1002/jctb.6721.
[40] H. Park and I.-G. Choi, “Genomic and transcriptomic perspectives on mycoremediation of polycyclic aromatic hydrocarbons,” Appl Microbiol Biotechnol, vol. 104, no. 16, pp. 6919–6928, 2020, doi: 10.1007/s00253-020-10746-1.
[41] F. Song, Y. Shi, S. Jia, Z. Tan, and H. Zhao, “Advances of naphthalene degradation in Pseudomonas putida ND6”, AIP Conf Proc, vol. 1944, no. 1, p. 020074, Mar. 2018, doi: 10.1063/1.5029792.
[42] B. Mohapatra and P. S. Phale, “Microbial Degradation of Naphthalene and Substituted Naphthalenes: Metabolic Diversity and Genomic Insight for Bioremediation”, Front Bioeng Biotechnol, vol. 9, p. 144, Mar. 2021, doi: 10.3389/FBIOE.2021.602445/BIBTEX.
[43] A. K. Shankhwar and R. Paliwal, “Fungi in bioremediation of soil: Mycoremediation of PAHs compounds”, Fungi Bio-prospects in Sustainable Agriculture, Environment and Nano-technology: Volume 2: Extremophilic Fungi and Myco-mediated Environmental Management, pp. 157–170, Jan. 2021, doi: 10.1016/B978-0-12-821925-6.00008-3.
[44] S. C. Chun, M. Muthu, N. Hasan, S. Tasneem, and J. Gopal, “Mycoremediation of PCBs by Pleurotus ostreatus: Possibilities and Prospects”, Applied Sciences, vol. 9, no. 19, p. 4185, Oct. 2019, doi: 10.3390/app9194185.
[45] E. Azin, K. Jenab, and H. Moghimi, “Biodegradation of crude oil by a halotolerant and biosurfactant producing strain of Mucor circinelloides in different microcosm conditions,” https://doi.org/10.1080/03067319.2020.1828385, 2020, doi: 10.1080/03067319.2020.1828385.
[46] E. Azin and H. Moghimi, “Efficient mycosorption of anionic azo dyes by Mucor circinelloides: Surface functional groups and removal mechanism study”, J Environ Chem Eng, vol. 6, no. 4, pp. 4114–4123, Aug. 2018, doi: 10.1016/J.JECE.2018.06.002.
