Evaluación de la citotoxicidad de andamios de PCL y PLGA con células troncales de pulpa dental para ingeniería tisular
Palabras clave:
Células troncales de pulpa dental, poli-ε-caprolactona. ácido poli-láctico-co-glicólico, andamios, ingeniería tisular.Resumen
Materiales de diferente naturaleza como poli- ε caprolactona y el ácido poli(láctico-co-glicólico), han sido empleados en medicina regenerativa con aplicaciones en humanos y, las células troncales de pulpa dental representan una fuente atractiva para emplearse en ensayos in vitro debido a su fácil acceso, buena viabilidad celular ya que se obtienen de terceros molares humanos en una extracción de rutina.
Objetivo: Evaluar la citotoxicidad in vitro de andamios de PCL/PLGA cultivados con células troncales de pulpa dental.
Materiales y métodos: Se realizó un estudio experimental in vitro evaluando los andamios de PCL/PLGA en dos proporciones (80:20 y 90:10, respectivamente) a los cuales se les sembraron células troncales de pulpa dental obtenidas de terceros molares. La viabilidad celular se evaluó a tres tiempos 3, 7 y 10 días mediante el ensayo MTT. El análisis de los datos se realizó con el software Stata versión 14 y la prueba "t" de Student.
Resultados: Se logró cultivar y caracterizar las células troncales de pulpa dental. Los resultados mostraron un aumento de la viabilidad celular a partir del día 3 en el grupo control y ambas mezclas de polímeros, pero existió una menor actividad metabólica en la mezcla PCL/PLGA (90:10) respecto a la mezcla PCL/PLGA (80:20).
Conclusiones: La mezcla PCL/PLGA 80:20 tuvo mayor viabilidad celular en los días 7 y 10 comparada con la PCL/PLGA de 90:10. También se demostró que ninguna de las dos mezclas tiene efectos citotóxicos en las células troncales de pulpa dental.
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